Anti-Histone H3 (di methyl K18) antibody [RM168] (ab214726)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM168] to Histone H3 (di methyl K18)
- Suitable for: Dot blot, ELISA, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H3 (di methyl K18) antibody [RM168]
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [RM168] to Histone H3 (di methyl K18) -
Host species
Rabbit -
Specificity
ab214726 reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18, monomethylated Lysine 18 (K18me1), or other methylations in histone H3. -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- Acid extracts of HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM168 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H3 (di methyl K18) antibody [RM168] (ab214726) at 0.5 µg/ml
Lane 1 : recombinant Histone H3.3
Lane 2 : acid extracts of HeLa cells
Predicted band size: 15 kDa
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A Peptide dotblot shows ab214726 only reacts to Histone H3 dimethyl K18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2) or dimethylated Lysine 36 (K36me2).
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ab214726 specifically reacts to Histone H3 dimethylated
at Lysine 18 (K18me2). No cross reactivity with other
methylated lysines in Histone H3.
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