Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K9) - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K9) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K9) - ChIP Grade -
Host species
Rabbit -
Specificity
In Dot blot detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H3 aa 1-12 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:ARTKQTAR(Ac)KSTG-C
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Positive control
- ChIP: U2OS cells, Human Cell lysate - nuclear (Primary CD34+ cells) WB: Mouse MEL cell nuclear lysate, HeLa cell acid-extract
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General notes
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4441 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) This image is courtesy of an Abreview by Brian Segan.ChIP analysis using unpurified ab4441 binding Histon H3 in human primary CD34+ cell lysate. Cells were cross-linked for 15 minutes with 1% formaldehyde. Samples were incubated with primary antibody (0.2 µg/µg of chromatin) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
Positive controls:
Region upstream of the transcription start site of the ACTB gene.
Region upstream of the transcription start site of the EGR1 gene.Negative Controls:
Region on chromosome 12 (Untr12) that is far from any known gene annotation and not expected to be bound by Histone H3 (acetyl K9). -
Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) This image is courtesy of an anonymous Abreview.All lanes : Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) at 1/2500 dilution
Lane 1 : Mouse MEL cell nuclear lysate
Lane 2 : Mouse MEL cell nuclear lysate treated with 0.4 µM Trichostatin A treatment for 18 hours.
Lysates/proteins at 9 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
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Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)All lanes : Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) at 0.5 µg/ml
Lane 1 : Untreated HeLa cell acid-extract
Lane 2 : HeLa cell acid-extract treated with sodium butyrateab4441, Histone H3, acetylated (Lys9) Pab Rabbit x Human
