Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM238] to Histone H2B (phospho S14)
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-Histone H2B (phospho S14) antibody [RM238]
See all Histone H2B primary antibodies
Description

Rabbit monoclonal [RM238] to Histone H2B (phospho S14)
Host species

Rabbit
Specificity

ab222762 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Histone H2B (phospho S14).
Positive control
- WB: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole. ICC/IF: HeLa cells.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

ab222762 was purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM238
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2B (phospho S14) (red) using ab222762 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green). DAPI nuclear stain (blue).
Western blot - Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

All lanes : Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762) at 0.5 µg/ml

Lane 1 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
Lane 2 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole

Predicted band size: 14 kDa
Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com