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Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free
    See all Histone H2B primary antibodies
  • Description

    Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Histone H2B acetylated on Lysine 15.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247351 is the carrier-free version of ab62335. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247351 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP955Y
  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
    Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
    All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

    Lane 1 : HeLa cell lysate (untreated)
    Lane 2 : HeLa cell lysate (treated with TSA)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab62335, the same antibody clone in a different buffer formulation.

    The bottom image shows beta Tubulin as a positive control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
    This data was developed using ab62335, the same antibody clone in a different buffer formulation.Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
  • Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
    Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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