Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578)
Key features and details
- Rabbit polyclonal to Histone H2A (acetyl K5) - N-terminal
- Suitable for: Dot blot, ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2A (acetyl K5) antibody - N-terminal
See all Histone H2A primary antibodies -
Description
Rabbit polyclonal to Histone H2A (acetyl K5) - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Histone H2A (N terminal) (acetyl K5). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P04908 -
Positive control
- WB: HeLa treated with 0.4 µM TSA, whole cell lysate. IP: HeLa treated with 0.4 µM TSA, whole cell lysate. ICC/IF: HeLa treated with 0.4 µM TSA cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578)All lanes : Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.012% DMSO for 18 hours, whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.4 µM Trichostatin A for 18 hours, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Dot Blot - Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578)Dot blot analysis of Histone H2A (acetyl K5) labeled with ab229578 at 1/500 dilution.
Lane 1: H2A.2K5un peptide.
Lane 2: H2A.2K5ac peptide.
Lane 3: H2BK20un peptide.
Lane 4: H2BK20ac peptide.
Lane 5: H2BK5un peptide.
Lane 6: H2BK5ac peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells (green) untreated (left panels) and treated with 0.4 μM TSA for 18 hours (right panels) stained for Histone H2AK5ac (acetyl Lys5) using ab229578 at 1/500 dilution.
Nucelar counterstain: Hoechst 33342 (blue).
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Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody - N-terminal (ab229578)Histone H2A (acetyl K5) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 0.4 µM TSA for 18 hours, whole cell lysate with 2.5 μg of ab229578. Western blot was performed from the immunoprecipitate using ab229578 at 1/10000 dilution.
Lane 1: Histone H2A (acetyl K5) expressing HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate 30 μg (Input).
Lane 2: 2.5 μg of pre-immune rabbit IgG instead of ab229578 in HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate.
Lane 3: ab229578 IP in HeLa treated with 0.4 µM TSA for 18 hours, whole cell lysate.
