Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] - BSA and Azide free (ab251032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2 - BSA and Azide free
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] - BSA and Azide free
See all Hepcidin + Hepcidin-2 primary antibodies -
Description
Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251032 is the carrier-free version of ab190775. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251032 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR18937 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocytes of Mouse liver was observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/200 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 20 secondsThis data was developed using ab190775, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Highly expressed in the liver and to a much lesser extent in the heart (PMID: 11113132 and 12729891).
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All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/5000 dilution
Lane 1 : Mouse Hepcidin recombinant fragment protein
Lane 2 : Mouse Hepcidin-2 recombinant fragment protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?This data was developed using ab190775, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.
The mouse recombinant fragment proteins contain aa24-83 with a GST-Tag and were made in-house.
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized frozen section of Mouse liver labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic staining on hepatocytes of Mouse liver.Counter stained with DAPI.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse kidney. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse spleen. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Rat liver. This could be because the ab does not XR with Rat hepcidin (77% identity between mouse and rat hapcidin sequences). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab190775, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon cancer. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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