Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Hemoglobin subunit alpha antibody [EPR3608]
See all Hemoglobin subunit alpha primary antibodies
Description

Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha
Host species

Rabbit
Specificity

This antibody gave a positive signal in Western blotting of rat brain, heart, kidney and spleen tissue. This antibody works in human, mouse and rat in IHC.

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 50-150.
(Peptide available as ab206105)
Positive control
- K562 cell lysate, human heart lysate, human spleen tissue, human stomach cancer tissue, human lung tissue, human placenta, rat heart lysate, mouse spleen lysate and mouse cerebral cortex.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3608
Isotype

IgG
Research areas

Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/2000 dilution (purified)

Lane 1 : Human heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/10000 dilution (purified) + Mouse spleen lysate at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/1000 dilution (unpurified)

Lane 1 : K562 cell lysate
Lane 2 : Human heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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