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Cardiovascular Blood Serum Proteins

Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hemoglobin subunit alpha antibody [EP3607]
    See all Hemoglobin subunit alpha primary antibodies
  • Description

    Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha
  • Host species

    Rabbit
  • Specificity

    Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562,fetal liver, and fetal heart lysates IHC-P: Human spleen and Human colon tissues
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP3607
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other

Images

  • Western blot - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
    Western blot - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
    All lanes : Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : Fetal heart lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human spleen tissue, using ab109444 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded human colon tissue, using ab109444 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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