Anti-HDAC7 antibody (ab12174)
Key features and details
- Rabbit polyclonal to HDAC7
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC7 antibody
See all HDAC7 primary antibodies -
Description
Rabbit polyclonal to HDAC7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
KPRLRQIPSAEDLETDG
conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. -
Positive control
- WB: HeLa and NIH3T3 cell lysates IP: HEK-293T cell lysates
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC7 antibody (ab12174)All lanes : Anti-HDAC7 antibody (ab12174) at 1/500 dilution
Lane 1 : HeLa nuclear lysate
Lane 2 : NIH3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 103 kDa
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Immunoprecipitation - Anti-HDAC7 antibody (ab12174)HDAC7 was immunoprecipitated from whole cell extracts of human HEK-293T cells expressing HDAC7-FLAG using ab12174. The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® and developed using Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate.
Lane 1: 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of HEK-293T cells expressing HDAC7-FLAG
Lane 2: Negative control (extract without antibody).
