Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-HDAC6 antibody [EPR22951-29] (ab239362)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22951-29] to HDAC6
Suitable for: WB, Flow Cyt, IP
Reacts with: Mouse, Rat

Product name

Anti-HDAC6 antibody [EPR22951-29]
See all HDAC6 primary antibodies
Description

Rabbit monoclonal [EPR22951-29] to HDAC6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat brain lysate. NIH/3T3, Neuro-2a and PC-12 lysates. Flow Cyt: Neuro-2a and NIH/3T3 cells. IP: Mouse brain lysate and NIH/3T3 lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22951-29
Isotype

IgG
Research areas

Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 131 kDa
Observed band size: 130 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

Exposure time: Lane 1-2: 32 seconds Lane 3: 3 minutes.
Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 131 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Kidney is a low-expression tissue.

A positive unidentified band between 15-20 kDa is detected in mouse kidney lysate.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

Exposure time: 48 seconds.
Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

HDAC6 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10µg

Lane 2: ab239362 IP in NIH/3T3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

HDAC6 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain lysate 10µg

Lane 2: ab239362 IP in mouse brain lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in mouse brain lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 mins.
Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.
Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.
Anti-HDAC6 antibody [EPR22951-29] (ab239362)

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