Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC6 antibody [EPR22951-29] (ab239362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22951-29] to HDAC6
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-HDAC6 antibody [EPR22951-29]
    See all HDAC6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22951-29] to HDAC6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysate. NIH/3T3, Neuro-2a and PC-12 lysates. Flow Cyt: Neuro-2a and NIH/3T3 cells. IP: Mouse brain lysate and NIH/3T3 lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 131 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026). 

    Exposure time: Lane 1-2: 32 seconds Lane 3: 3 minutes.

  • Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    All lanes : Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 131 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Kidney is a low-expression tissue.

    A positive unidentified band between 15-20 kDa is detected in mouse kidney lysate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

    Exposure time: 48 seconds.

  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    HDAC6 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10µg

    Lane 2: ab239362 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    HDAC6 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10µg

    Lane 2: ab239362 IP in mouse brain lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

     

  • Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Flow Cytometry - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Anti-HDAC6 antibody [EPR22951-29] (ab239362)
    Anti-HDAC6 antibody [EPR22951-29] (ab239362)

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