Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1698(2)] to HDAC6 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human, African green monkey

Overview

  • Product name

    Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free
    See all HDAC6 primary antibodies

  • Description

    Rabbit monoclonal [EPR1698(2)] to HDAC6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HAP1, Jurkat, K562, and COS-1 cell lysates, human kidney tissue.

  • General notes

    ab210472 is the carrier-free version of ab133493.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HDAC6 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 131 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab133493).

      Lanes 1- 2: Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab133493 was shown to react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264804 (knockout cell lysate ab257145) was used. Wild-type HeLa and HDAC6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Immunocytochemistry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

    Clone EPR1698(2) (ab210472) has been successfully conjugated by Abcam. This image was generated using Anti-HDAC6 antibody [EPR1698(2)] (Alexa Fluor® 647). Please refer to ab202833 for protocol details.

    ab202833 staining HDAC6 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202833 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Immunocytochemistry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

    Clone EPR1698(2) (ab210472) has been successfully conjugated by Abcam. This image was generated using Anti-HDAC6 antibody [EPR1698(2)] (Alexa Fluor® 488). Please refer to ab202948 for protocol details.

    ab202948 staining HDAC6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202948 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Lanes 1-2 : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilution
    Lanes 3-4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution


    Lanes 1 & 3 & 5 : Wild-type HAP1 cell lysate
    Lanes 2 & 4 & 6 : HDAC6 knockout HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 131 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab133493).

    Lanes 1 and 2: Green signal from target – ab133493 observed at 160 kDa
    Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab133493 was shown to specifically react with HDAC6 when HDAC6 knockout samples were used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. ab133493 and ab8226 (loading control to beta actin) were diluted 1/10 000 and 1/1000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Flow Cytometry - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

    ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133493).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HDAC6 with ab133493 antibody at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133493).

  • Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
    Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

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