Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18473] to KAT7 / Hbo1 / MYST2
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473]
See all KAT7 / Hbo1 / MYST2 primary antibodies
Description

Rabbit monoclonal [EPR18473] to KAT7 / Hbo1 / MYST2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, MCF7, NIH/3T3, Jurkat, C6 and RAW 264.7 cell lysates; human breast cancer lysate. IHC-P: human colon, human testis, mouse colon, rat colon and rat testis tissues. ICC/IF: HEK-293 cells. IP: Jurkat whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18473
Isotype

IgG
Research areas

Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 71 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution

Lane 1 : C6 (Rat glial tumor cells) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/1000 dilution + human breast cancer lysate at 40 µg

Secondary
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HEK-293 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab190908 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary at 1/500 dilution.
Immunoprecipitation - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab190908 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab190908 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Jurkat whole cell lysate 10ug (Input).

Lane 2: ab190908 IP in Jurkat whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190908 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)

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