Anti-SNRPD2 antibody [EPR16762] (ab198296)
![Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-239969-anti-sm-d2-antibody-epr16762-western-blot.jpg "Anti-SNRPD2 antibody [EPR16762] (ab198296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16762] to SNRPD2
- Suitable for: WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SNRPD2 antibody [EPR16762]
See all SNRPD2 primary antibodies -
Description
Rabbit monoclonal [EPR16762] to SNRPD2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P RatHumanIP HumanWB MouseHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, MCF7, A549, HeLa, Mouse brain, Mouse spleen, Rat brain, Mouse spleen, C6, RAW 264.7 and PC12 lysates. IHC: Human and Rat kidney tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells
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General notes
This product was previously labelled as Sm-D2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16762 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)All lanes : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-239971-anti-sm-d2-antibody-epr16762-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-239970-anti-sm-d2-antibody-epr16762-western-blot.jpg)
Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)Lanes 1-6 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/2000 dilution
Lane 7 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-239972-anti-sm-d2-antibody-epr16762-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.
Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-246175-anti-sm-d2-antibody-epr16762-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] (ab198296)NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2: IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Flow Cytometry - Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-263972-anti-sm-d2-antibody-epr16762-flow-cytometry.jpg)
Flow Cytometry - Anti-SNRPD2 antibody [EPR16762] (ab198296)Flow cytometry analysis of HeLa cells labelling SNRPD2 (red) with purified ab198296 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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![Anti-SNRPD2 antibody [EPR16762] (ab198296)](https://www.abcam.com/ps/products/198/ab198296/Images/ab198296-9-benefits-of-recombinant-antibodies.png)
Anti-SNRPD2 antibody [EPR16762] (ab198296)
