Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SNRPD2 antibody [EPR16762] (ab198296)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16762] to SNRPD2
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SNRPD2 antibody [EPR16762]
See all SNRPD2 primary antibodies
Description

Rabbit monoclonal [EPR16762] to SNRPD2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, MCF7, A549, HeLa, Mouse brain, Mouse spleen, Rat brain, Mouse spleen, C6, RAW 264.7 and PC12 lysates. IHC: Human and Rat kidney tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells
General notes
This product was previously labelled as Sm-D2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16762
Isotype

IgG
Research areas

Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)

All lanes : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SNRPD2 antibody [EPR16762] (ab198296)

Lanes 1-6 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/2000 dilution
Lane 7 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] (ab198296)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] (ab198296)

NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2: IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-SNRPD2 antibody [EPR16762] (ab198296)

Flow cytometry analysis of HeLa cells labelling SNRPD2 (red) with purified ab198296 at dilution of 1/150. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Anti-SNRPD2 antibody [EPR16762] (ab198296)

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