Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23173-30] to hSET1/SET1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-hSET1/SET1 antibody [EPR23173-30]
    See all hSET1/SET1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23173-30] to hSET1/SET1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and T47D lysates. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23173-30

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)
    Western blot - Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)
    All lanes : Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 186 kDa
    Observed band size: 300 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 3 minutes ; Lane 2: 37 seconds.

    Fresh lysates are recommended to run WB in avoiding to degradation for this target.

     

  • Flow Cytometry - Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)
    Flow Cytometry - Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling hSET1/SET1 with ab243639 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)
    Anti-hSET1/SET1 antibody [EPR23173-30] (ab243639)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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