Anti-HDAC6 antibody [159] (ab253033)
![Anti-HDAC6 antibody [159] (ab253033)](https://www.abcam.com/ps/products/253/ab253033/Images/ab253033-343580-anti-hdac6-antibody-immunocytochemistry-hek293t-human.jpg "Anti-HDAC6 antibody [159] (ab253033)")
Key features and details
- Mouse monoclonal [159] to HDAC6
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody [159]
See all HDAC6 primary antibodies -
Description
Mouse monoclonal [159] to HDAC6 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human HDAC6.
Database link: Q9UBN7 -
Epitope
The antibody detects epitope in SE domain of HDAC6 (region AA 971-981) -
Positive control
- ICC/IF: HEK-293T and SH-SY5Y cells. WB: HDAC6 recombinant protein.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called A1488 Anti-HDAC6 Monoclonal Antibody (SE domain-specific).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Affinity purified from hybridoma culture supernatant. -
Clonality
Monoclonal -
Clone number
159 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [159] (ab253033)
Formaldehyde-fixed, Triton-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells (A) and SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (B) stained for HDAC6 (Green) using ab253033 at 25 µg/mL in ICC/IF analysis. An anti-tubulin antibody was used to countertsain tubulin (Red) and the nuclear counterstain was DAPI (Blue). The secondary antibodies were an anti-mouse antibody conjugated to AlexFluor®488 and AlexaFluor®647 respectively.
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![Western blot - Anti-HDAC6 antibody [159] (ab253033)](https://www.abcam.com/ps/products/253/ab253033/Images/ab253033-343581-anti-hdac6-antibody-westernblot-human.jpg)
Western blot - Anti-HDAC6 antibody [159] (ab253033)All lanes : Anti-HDAC6 antibody [159] (ab253033) at 1 µg/ml
Lane 1 : Recombinant human HDAC6 priotein (aa 844-1112) protein, 80 ng
Lane 2 : Recombinant human HDAC6 protein (aa 2-1215) protein, 80 ng
Secondary
All lanes : anti-mouse IgG HRP
Predicted band size: 131 kDaMembrane was blocked with 5% milk.
The secondary antibody was a HRP-conjugated ant-mouse IgG.
The experiment was conducted under reducing conditions using PBS/0.05% Tween-20 Buffer.
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![ELISA - Anti-HDAC6 antibody [159] (ab253033)](https://www.abcam.com/ps/products/253/ab253033/Images/ab253033-343582-anti-hdac6-antibody-elisa-human.jpg)
ELISA - Anti-HDAC6 antibody [159] (ab253033)Detection of Human HDAC6 using ab253033 by ELISA. The BSA-blocked plate was probed with a diltuion series of ab253033 (10-6 - 102 nM) followed by incubation with an anti-mouse IgG secondary antibody conjugated to HRP.