Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ki67 antibody [SP6], prediluted (ab21700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP6] to Ki67, prediluted
  • Suitable for: WB, ICC, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Ki67 antibody [SP6], prediluted
    See all Ki67 primary antibodies

  • Description

    Rabbit monoclonal [SP6] to Ki67, prediluted

  • Host species

    Rabbit

  • Specificity

    ab21700 recognises Ki67.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos and HeLa cell lysates. IHC-P: Human tonsil and thymus tissue; Human endometrial and breast adenocarcinoma tissue. ICC: HeLa and HAP1 cells. Flow Cyt:: HAP1 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product was switched from hybridoma to recombinant format on 25th October 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Ki67 with ab21700.
  • Western blot - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Western blot - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    All lanes : Anti-Ki67 antibody [SP6] (ab16667) at 1/100 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Wild-type HeLa cell lysate
    Lane 3 : MKI67 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 358 kDa
    Observed band size: 359 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab16667).

    Lanes 1 - 3: Merged signal (red and green). Green - ab16667 observed at 359 kDa. Red - loading control, ab130007 observed at 125 kDa.  

     ab16667 was shown to react with Ki67 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255407 (knockout cell lysate ab263762) was used. Wild-type and Ki67 knockout samples were subjected to SDS-PAGE. ab16667 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 100 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, None permeabilized parental HAP1 cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in parental HAP1cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

  • Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, None permeabilized HeLa cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).

  • Flow Cytometry - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Flow Cytometry - Anti-Ki67 antibody [SP6], prediluted (ab21700)

    This data was developed using ab16667, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) cells labelling Ki67 with ab16667 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial adenocarcinoma tissue, staining Ki67 with ab21700.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining Ki67 with ab21700.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast adenocarcinoma tissue, staining Ki67 with ab21700.
  • Anti-Ki67 antibody [SP6], prediluted (ab21700)
    Anti-Ki67 antibody [SP6], prediluted (ab21700)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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