Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [37C7-12] to Ki67 - BSA and Azide free
  • Suitable for: ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Ki67 antibody [37C7-12] - BSA and Azide free
    See all Ki67 primary antibodies

  • Description

    Mouse monoclonal [37C7-12] to Ki67 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast carcinoma and tonsil tissue. ICC: HeLa and HAP1 cells.

  • General notes

    ab252260 is the carrier-free version of ab245113. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ki67 with ab245113 at 1.133µg/ml, followed by a ready to use secondary. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation  PBS, sodium azide, glycerol and BSA (ab245113).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ki67 with ab245113 at 1.133µg/ml, followed by a ready to use secondary. Nuclear staining on human tonsil tissue is observed. The section was incubated with ab245113 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

  • Immunocytochemistry - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
    Immunocytochemistry - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Ki67 with ab245113 at 9.06µg/ml, followed by a Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

  • Immunocytochemistry - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
    Immunocytochemistry - Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HAP1 (human chronic myelogenous leukemia near-haploid cell line) cells labeling Ki67 with ab245113 at 9.06µg/ml, followed by a Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolus staining on HAP1 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab245113 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab245113, PBS, sodium azide, glycerol and BSA.

  • Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)
    Anti-Ki67 antibody [37C7-12] - BSA and Azide free (ab252260)

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