Anti-HA Affinity Resin - Amintra (ab270603)
Overview
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Product name
Anti-HA Affinity Resin - Amintra -
Description
Anti-HA Affinity Resin - Amintra -
General notes
Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.
The HA tag is derived from the human influenza virus HA protein corresponding to amino acids 98-106 (YPYDVPDYA). The HA tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein, so it has been extensively used as a general epitope tag at the N-terminal or C-terminal of the fusion protein. Anti-HA Affinity Resin - Amintra is based on a 4% agarose beads matrix, and non-specific binding is rare. It can be used for HA fusion protein purification and immunoprecipitation.
The characteristics of the resin:
Item
Description
Matrix
4% agarose beads
Ligand
Anti-HA Antibody
Binding capacity
>1mg HA fusion protein/mL medium
Particle Size (μm)
45–165
Max. Pressure
0.1 MPa, 1 bar
Storage buffer
1 x PBS with 0.02% sodium azide
Storage Temperature
4ºC
For the purification of HA-tagged fusion proteins, Amintra Anti-HA Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.
When a small amount of sample is being purified, immunoprecipitation procedure is recommended.
Notes:
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Anti-HA Affinity Beads. Expedeon product codes AHA0001 (1 mL medium) and AHA0005 (5 mL medium) are the same as the current sizes: 1 mL and 5 mL, respectively.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 6
Preservative: 0.02% Sodium azide
Constituent: PBS -
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