Immunohistochemistry analysis of Mouse backskin tissue using Mouse Anti-Hsp27 Monoclonal Antibody, at 1:100 dilution for 1 hour at RT.  FITC Goat Anti-Mouse (green) was used as a Secondary Antibody at 1:50  dilutionfor 1 hour. Localization: Epidermis.

Anti-Hsp27 antibody [8A7] (ab79868) at 1/1000 dilution + Rat lung tissue lysate

Predicted band size: 23 kDa

ab79868 at 1/100,000 dilution staining Hsp27 in human colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining.

ab79868 (1/100) staining Hsp27 in Mouse epidermis tissue sections by IF (Iimmunofluorescence). Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

ab79868 staining Hsp27 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).

Overlay histogram showing HeLa cells stained with ab79868 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79868, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.