Anti-GW182 antibody (ab110233)
Key features and details
- Rabbit polyclonal to GW182
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GW182 antibody
See all GW182 primary antibodies -
Description
Rabbit polyclonal to GW182 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in HeLa cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseRatRabbitHorseCowDogChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes ICC/IF (1) Use a concentration of 1 µg/ml.Notes ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies. - Information by UniProt
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Database links
- Entrez Gene: 27327 Human
- Entrez Gene: 233833 Mouse
- Entrez Gene: 308971 Rat
- Omim: 610739 Human
- SwissProt: Q8NDV7 Human
- SwissProt: Q3UHK8 Mouse
- Unigene: 655057 Human
- Unigene: 102305 Mouse
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Alternative names
- CAG repeat protein 26 antibody
- CAGH26 antibody
- DKFZp666E117 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GW182 antibody (ab110233)ICC/IF image of ab110233 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 cells at 1µg/ml.
Protocols
Datasheets and documents
References (1)
ab110233 has been referenced in 1 publication.
- Gallego-Iradi MC et al. Subcellular Localization of Matrin 3 Containing Mutations Associated with ALS and Distal Myopathy. PLoS One 10:e0142144 (2015). PubMed: 26528920
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GW182 antibody (ab110233)ICC/IF image of ab110233 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 cells at 1µg/ml.
