Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19969] to GSTA1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GSTA1 antibody [EPR19969] - BSA and Azide free
    See all GSTA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19969] to GSTA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251473 is the carrier-free version of ab207413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251473 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution

    Lane 1 : Human GSTA1 full length recombinant protein
    Lane 2 : Human GSTA2 full length recombinant protein
    Lane 3 : Human GSTA3 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 5 seconds


    This data was developed using ab207413, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    GSTA1, A2, and A3 are His-Tagged full-length (aa1-222) recombinant proteins.

    GSTA4 and A5 were not recognized by ab207413 (data not shown).

  • Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    This data was developed using ab207413, the same antibody clone in a different buffer formulation.GSTA1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HepG2 whole cell lysate, 10µg (Input). Lane 2: ab207413 IP in HepG2 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab207413 in HepG2 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    This data was developed using ab207413, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 3 minutes; Lanes 5 and 6: 1 second.

  • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 minutes


    This data was developed using ab207413, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
    Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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