Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3014(2)] to GGA1 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free
    See all GGA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3014(2)] to GGA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249621 is the carrier-free version of ab170956. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249621 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3014(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)

    This data was developed using ab170956, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: GGA1 knockout HAP1 cell lysate (20 µg) 

    Lane 3: Jurkat cell lysate (20 µg)

    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    This data was developed using ab170956, the same antibody clone in a different buffer formulation.Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.
  • Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    All lanes : Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    This data was developed using ab170956, the same antibody clone in a different buffer formulation.

     

  • Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
    Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)

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