Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
![Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)](https://www.abcam.com/ps/products/249/ab249621/Images/ab249621-406433-anti-gga1-antibody-epr3014-2-bsa-and-azide-free-western-blot.jpg "Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3014(2)] to GGA1 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free
See all GGA1 primary antibodies -
Description
Rabbit monoclonal [EPR3014(2)] to GGA1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249621 is the carrier-free version of ab170956. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249621 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3014(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
This data was developed using ab170956, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GGA1 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)](https://www.abcam.com/ps/products/249/ab249621/Images/ab249621-2-anti-gga1-antibody-epr3014-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621) -
![Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)](https://www.abcam.com/ps/products/249/ab249621/Images/ab249621-398698-anti-gga1-antibody-epr3014-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)All lanes : Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDaThis data was developed using ab170956, the same antibody clone in a different buffer formulation.
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![Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)](https://www.abcam.com/ps/products/249/ab249621/Images/ab249621-4-benefits-of-recombinant-antibodies.png)
Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (ab249621)
