Anti-TOMM22/TOM22 antibody (ab246862)
Key features and details
- Rabbit polyclonal to TOMM22/TOM22
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TOMM22/TOM22 antibody
See all TOMM22/TOM22 primary antibodies -
Description
Rabbit polyclonal to TOMM22/TOM22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey
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Immunogen
Recombinant fragment corresponding to Human TOMM22/TOM22 aa 1-100.
Database link: Q9NS69 -
Positive control
- WB: NIH/3T3, NBT-II, U-2 OS and HL-60 whole cell lysates. IHC-P: Human pancreas, testis, fallopian tube and duodenum tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TOMM22/TOM22 antibody (ab246862)Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Predicted band size: 16 kDa
Western blot analysis in human cell line HL-60. -
Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody (ab246862)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TOMM22/TOM22 (green) using ab246862 at 4 μg/ml in ICC/IF.
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Western blot - Anti-TOMM22/TOM22 antibody (ab246862)All lanes : Anti-TOMM22/TOM22 antibody (ab246862) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) transfected with control siRNA, whole cell lysate
Lane 2 : U-2 OS transfected with target-specific siRNA #1, whole cell lysate
Lane 3 : U-2 OS transfected with target-specific siRNA #2, whole cell lysate
Predicted band size: 16 kDaRemaining relative intensity is presented. Loading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)Paraffin-embedded human duodenum tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)Paraffin-embedded human fallopian tube tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)Paraffin-embedded human testis tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody (ab246862)Paraffin-embedded human pancreas tissue stained for TOMM22/TOM22 using ab246862 at 1/200 dilution in immunohistochemical analysis.