Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13505] to TOMM22/TOM22 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal
    See all TOMM22/TOM22 primary antibodies

  • Description

    Rabbit monoclonal [EPR13505] to TOMM22/TOM22 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TOMM22/TOM22 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NS69

  • Positive control

    • HeLa, K562, Raji and 293T cell lysates. Kidney and liver tissue. Raji and 293T cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as TOMM22.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    All lanes : Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    Immunofluorescent analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Flow Cytometry - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    Flow cytometric analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    Western blot analysis on immunoprecipitation pellet from Raji cell lysate using ab179826 at 1/10 dilution for IP.

  • Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
    Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

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