Anti-GFI1 antibody (ab21061)
Key features and details
- Rabbit polyclonal to GFI1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GFI1 antibody -
Description
Rabbit polyclonal to GFI1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis
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Immunogen
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Positive control
- THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab21061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa). Target
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Function
Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription. -
Involvement in disease
Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Domain
The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2672 Human
- Entrez Gene: 14581 Mouse
- Entrez Gene: 24388 Rat
- Omim: 600871 Human
- SwissProt: Q99684 Human
- SwissProt: P70338 Mouse
- SwissProt: Q07120 Rat
- Unigene: 73172 Human
see all -
Alternative names
- GFI 1 antibody
- GFI1 antibody
- GFI1_HUMAN antibody
see all
Images
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Western blot - Anti-GFI1 antibody (ab21061)All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml
Lane 1 :THP-1 whole cell lysate (ab7913)
Lane 2 :THP-1 whole cell lysate (ab7913) with GFI1 peptide (ab22461) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible cleavage fragment), 36 kDa (possible cross reactivity)ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.
References (8)
ab21061 has been referenced in 8 publications.
- Weiss CN & Ito K microRNA-22 promotes megakaryocyte differentiation through repression of its target, GFI1. Blood Adv 3:33-46 (2019). PubMed: 30617215
- Lee C et al. Lsd1 as a therapeutic target in Gfi1-activated medulloblastoma. Nat Commun 10:332 (2019). PubMed: 30659187
- Adamik J et al. XRK3F2 Inhibition of p62-ZZ Domain Signaling Rescues Myeloma-Induced GFI1-Driven Epigenetic Repression of the Runx2 Gene in Pre-osteoblasts to Overcome Differentiation Suppression. Front Endocrinol (Lausanne) 9:344 (2018). PubMed: 30008697
- Collombet S et al. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc Natl Acad Sci U S A 114:5792-5799 (2017). WB . PubMed: 28584084
- Moignard V et al. Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis. Nat Cell Biol 15:363-72 (2013). PubMed: 23524953
- Khandanpour C et al. The human GFI136N variant induces epigenetic changes at the Hoxa9 locus and accelerates K-RAS driven myeloproliferative disorder in mice. Blood 120:4006-17 (2012). Mouse . PubMed: 22932805
- D'Souza S et al. Gfi1 expressed in bone marrow stromal cells is a novel osteoblast suppressor in patients with multiple myeloma bone disease. Blood 118:6871-80 (2011). WB ; Mouse . PubMed: 22042697
- Dabrowska MJ et al. Loss of MicroRNA targets in the 3' untranslated region as a mechanism of retroviral insertional activation of growth factor independence 1. J Virol 83:8051-61 (2009). WB ; Human . PubMed: 19474094
Images
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Western blot - Anti-GFI1 antibody (ab21061)All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml
Lane 1 :THP-1 whole cell lysate (ab7913)
Lane 2 :THP-1 whole cell lysate (ab7913) with GFI1 peptide (ab22461) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible cleavage fragment), 36 kDa (possible cross reactivity)ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.