Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TopBP1 antibody (ab105109)

Key features and details

  • Rabbit polyclonal to TopBP1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab105109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rabbit
Horse
Cow
Dog
Xenopus laevis
Macaque monkey
Gorilla
Chinese hamster
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa).

Target

  • Function

    Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.

  • Tissue specificity

    Highly expressed in heart, brain, placenta, lung and kidney.

  • Sequence similarities

    Contains 8 BRCT domains.

  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues in response to X-ray irradiation.
    Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.

  • Cellular localization

    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA topoisomerase 2 binding protein 1 antibody
      • DNA topoisomerase 2-binding protein 1 antibody
      • DNA topoisomerase II binding protein 1 antibody
      • DNA topoisomerase II binding protein antibody
      • DNA topoisomerase II-beta-binding protein 1 antibody
      • DNA topoisomerase II-binding protein 1 antibody
      • DNA topoisomerase IIbeta binding protein 1 antibody
      • Hypothetical protein KIAA0259 [Fragment] antibody
      • KIAA0259 antibody
      • TOP2BP1 antibody
      • TOPB1_HUMAN antibody
      • TOPBP 1 antibody
      • TopBP1 antibody
      • Topoisomerase (DNA) II binding protein 1 antibody
      • Topoisomerase II binding protein 1 antibody
      see all

    Images

    • Western blot - Anti-TopBP1 antibody (ab105109)
      Western blot - Anti-TopBP1 antibody (ab105109)
      All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 171 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 120 kDa, 42 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute
    • Western blot - Anti-TopBP1 antibody (ab105109)
      Western blot - Anti-TopBP1 antibody (ab105109)This image is courtesy of an anonymous Abreview
      All lanes : Anti-TopBP1 antibody (ab105109) at 0.5 µg/ml

      All lanes : Mouse ES whole cell lysate

      Lysates/proteins at 200 µg per lane.

      Secondary
      All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 171 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?
      Additional bands at: 145 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)


      Exposure time: 30 seconds

      See Abreview

    References (2)

    Publishing research using ab105109? Please let us know so that we can cite the reference in this datasheet.

    ab105109 has been referenced in 2 publications.

    • Hirota T  et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell 47:645-659.e6 (2018). PubMed: 30393076
    • Vasileva A  et al. The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse. J Cell Sci 126:3927-38 (2013). PubMed: 23788429

    Images

    • Western blot - Anti-TopBP1 antibody (ab105109)
      Western blot - Anti-TopBP1 antibody (ab105109)
      All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 171 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 120 kDa, 42 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute
    • Western blot - Anti-TopBP1 antibody (ab105109)
      Western blot - Anti-TopBP1 antibody (ab105109) This image is courtesy of an anonymous Abreview
      All lanes : Anti-TopBP1 antibody (ab105109) at 0.5 µg/ml

      All lanes : Mouse ES whole cell lysate

      Lysates/proteins at 200 µg per lane.

      Secondary
      All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 171 kDa
      Observed band size: 200 kDa why is the actual band size different from the predicted?
      Additional bands at: 145 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)


      Exposure time: 30 seconds

      See Abreview

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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