Anti-hlgB antibody (ab190338)
Key features and details
- Rabbit polyclonal to hlgB
- Suitable for: WB
- Reacts with: Staphylococcus aureus, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-hlgB antibody -
Description
Rabbit polyclonal to hlgB -
Host species
Rabbit -
Specificity
Due to the high level of homology among the bi-component cytolysins, ab190338 demonstrates cross-reactivity to rHlg C, rLukF-PV and rLukS-PV . The immunogen contained a His-tag, therefore, proteins containing a His-epitope tag may also cross-react. This antibody is an IgG fraction of serum and therefore may have antibodies to other antigens including Staphylococcal antigens as a result of natural exposure and immune response. -
Tested Applications & Species
See all applications and species dataApplication Species WB Staphylococcus aureusRecombinant fragment
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Immunogen
Recombinant full length protein (His-tag) corresponding to hlgB aa 1 to the C-terminus. Immunogen species: Staphylococcus aureus
Database link: P0A075 -
Positive control
- Recombinant hlg B protein, culture supernatants of Staphylococcus aureus strains USA300 , Newman and NCTC 8325-4.
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General notes
av190338 can be used for detection of gamma-hemolysin secreted by S. aureus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-hlgB antibody (ab190338)All lanes : Anti-hlgB antibody (ab190338) at 0.5 µg/ml
Lane 1 : Culture supernatant of Staphylococcus aureus strain USA300
Lane 2 : Culture supernatant of Staphylococcus aureus strain Newman
Lane 3 : Culture supernatant of Staphylococcus aureus strain NCTC 8325-4
Lane 4 : Culture supernatant of Staphylococcus aureus strain exoprotein negative NCTC 8325-4
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Anti-rabbit IgG-AP conjugate
Predicted band size: 37 kDa
Observed band size: -
Western blot - Anti-hlgB antibody (ab190338)Anti-hlgB antibody (ab190338) at 0.5 µg/ml + Recombinant hlg B protein at 0.1 µg
Secondary
Anti-rabbit IgG-AP conjugate
Predicted band size: 37 kDa