Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [53/1] to GDF9 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GDF9 antibody [53/1] - BSA and Azide free
    See all GDF9 primary antibodies

  • Description

    Mouse monoclonal [53/1] to GDF9 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human ovary, ovary cancer, testis and epididymis tissue lysate. Human recombinant GDFP-9 protein. Flow Cyt: NIH:OVCAR-3 cells.

  • General notes

    ab273472 is the carrier-free version of ab273455. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    All lanes : Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution

    Lane 1 : Human ovary tissue lysate
    Lane 2 : Human ovary cancer tissue lysate
    Lane 3 : Human testis tissue lysate
    Lane 4 : Human epididymis tissue lysate
    Lane 5 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 52 kDa
    Observed band size: 57,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    This data was produced using the same clone but in a different formulation, ab273455.

    The doublet bands are the precursor form of GDF 9 and have been described in the previous literature. (PMID: 24438375, 32350111)
    Negative control: Brain (PMID: 22732078).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    Flow Cytometry - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

    This data was produced using the same clone but in a different formulation, ab273455.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labelling GDF 9 with ab273455 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. 

  • Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    Western blot - Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    Anti-GDF9 antibody [53/1] (ab273455) at 1/1000 dilution + Human recombinant GDF9 protein, 20ng

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 52 kDa


    Exposure time: 1 second


    This data was produced using the same clone but in a different formulation, ab273455.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)
    Anti-GDF9 antibody [53/1] - BSA and Azide free (ab273472)

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