Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMP7 antibody [EPR5897] (ab129156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5897] to BMP7
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-BMP7 antibody [EPR5897]
    See all BMP7 primary antibodies

  • Description

    Rabbit monoclonal [EPR5897] to BMP7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BMP7 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • WB: Rat and mouse kidney lysate, Fetal kidney, Human prostate and HT-1376 lysates; recombinant Osteogenic Growth Peptide IP: MCF7 whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/5000 dilution (purified)

    Lane 1 : Rat kidney lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Blocking/Diluting buffer 5% NFDM/TBST
  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (purified) + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 1/15 dilution

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Blocking/Diluting buffer 5% NFDM/TBST
  • Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)
    Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)

    Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).

    Lane 1 (input): MCF7 whole cell lysate 10μg

    Lane 2 (+): ab129156 + MCF7 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129156 in MCF7 whole cell lysate

    For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal kidney lysate
    Lane 2 : Human prostate lysate
    Lane 3 : HT-1376 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 49 kDa

  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
    Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified) + recombinant Human BMP7 (amino acids 316 - 431) at 0.01 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 49 kDa



    The recombinant peptide should give a band of approximately 13.7 kDa.
  • Anti-BMP7 antibody [EPR5897] (ab129156)
    Anti-BMP7 antibody [EPR5897] (ab129156)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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