Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GC1q R antibody [EPR8871] (ab131284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8871] to GC1q R
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GC1q R antibody [EPR8871]
    See all GC1q R primary antibodies

  • Description

    Rabbit monoclonal [EPR8871] to GC1q R

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GC1q R aa 200-300. The exact sequence is proprietary.
    Database link: Q07021

  • Positive control

    • WB: HeLa, 293T, Jurkat, and Raji cell lysates. Human, mouse and rat heart tissue lysates. IHC-P: Human kidney tissue and Human tonsil tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 0.01 µg/ml (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)
    OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GC1q R antibody [EPR8871] (ab131284)
    Anti-GC1q R antibody [EPR8871] (ab131284)

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