Anti-MHC Class II antibody [OX3] - BSA and Azide free (ab244596)
![Anti-MHC Class II antibody [OX3] - BSA and Azide free (ab244596)](https://www.abcam.com/ps/products/244/ab244596/Images/ab244596-1-ab243843MousemonoclonalOX3toMHCclassIILewisratsplenocytesflowcytometry.jpg "Anti-MHC Class II antibody [OX3] - BSA and Azide free (ab244596)")
Key features and details
- Mouse monoclonal [OX3] to MHC Class II - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-MHC Class II antibody [OX3] - BSA and Azide free
See all MHC Class II primary antibodies -
Description
Mouse monoclonal [OX3] to MHC Class II - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Rat splenocytes
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General notes
ab244596 is the carrier-free version of ab243843. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab244596 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX3 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-MHC Class II antibody [OX3] - BSA and Azide free (ab244596)
This data was developed using the same antibody clone in a different buffer formulation (ab243843).
Flow cytometry staining of Lewis rat splenocytes with ab243843 (right) or mouse IgG1 kappa (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab243843) or mouse IgG1 kappa (ab170190) isotype (1x106 in 100 μl at 0.008 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD45R PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live lymphocytes.