Anti-GATAD2B antibody (ab76925)
Key features and details
- Rabbit polyclonal to GATAD2B
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GATAD2B antibody
See all GATAD2B primary antibodies -
Description
Rabbit polyclonal to GATAD2B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human GATAD2B aa 50-100.
Database link: NP_065750.1 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76925 was affinity purified using an epitope specific to GATAD2B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GATAD2B antibody (ab76925)All lanes : Anti-GATAD2B antibody (ab76925) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (ab76925)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GATAD2B with ab76925 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-GATAD2B antibody (ab76925)ab76925, at 1µg/ml, staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
