Recombinant human DNMT3A + DNMT3L protein (ab198095)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human DNMT3A + DNMT3L protein -
Biological activity
Specific Activity: 0.58 pmol/min/µg.
Assay Conditions: Assayed using a Dnmt3a Assay Kit. Add 50 µl reaction mix containing Dnmt3a/Dnmt3L to wells coated with Dnmt substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.
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Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
YPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITV GMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKG LYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRF LESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHG RIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPV HYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV -
Predicted molecular weight
34 kDa including tags -
Amino acids
623 to 912 -
Tags
His tag N-Terminus -
Additional sequence information
Dnmt3a; GenBank Accession No. NM_175629.
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Amino Acid Sequence 2
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Species
Human -
Sequence
WRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFL ESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWY LFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTI PDVHGGSLQNAVRVWSNIPAIRSSRHWALVSEEELSLLAQNKQSSKLAAK WPTKLVKNCFLPLREYFKYFSTELTSSL -
Predicted molecular weight
52 kDa including tags -
Amino acids
160 to 387 -
Tags
GST tag N-Terminus -
Additional sequence information
Dnmt3L; GenBank Accession No. NM_013369
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Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Complex of active Human Dnmt3a, (NM_175629) aa 623-912 (end) with N terminal His tag, MW=34 kDa, and Dnmt3L (NM_013369) aa160-387 (end) with an N terminal GST tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
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Images
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Functional Studies - Recombinant human DNMT3A + DNMT3L protein (ab198095)
Activity assay using ab198095.
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SDS-PAGE - Recombinant human DNMT3A + DNMT3L protein (ab198095)10% SDS-PAGE analysis of 3 µg ab198095 with Coomassie staining.