Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23598-126] to GALC - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GALC antibody [EPR23598-126] - BSA and Azide free
    See all GALC primary antibodies

  • Description

    Rabbit monoclonal [EPR23598-126] to GALC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-12 whole cell lysate; Mouse brain and testis lysates; Rat brain and testis lysates; Human brain lysate. IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue.

  • General notes

    ab272174 is the carrier-free version of ab240638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240638).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240638).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240638).

  • Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)
    Anti-GALC antibody [EPR23598-126] - BSA and Azide free (ab272174)

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