Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17582-45] to TSG6 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free
    See all TSG6 primary antibodies

  • Description

    Rabbit monoclonal [EPR17582-45] to TSG6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours,) whole cell lysate; Rat amniotic membrane tissue lysate. Flow cyt: THP-1 (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) cells. IP: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate.

  • General notes

    ab274383 is the carrier-free version of ab267469. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution + Rat amniotic membrane tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 31 kDa
    Observed band size: 120,36 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

    The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469)

  • Flow Cytometry - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Flow Cytometry - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) (Red) / Untreated control (Green) cells labelling TSG6 with ab267469 at 1/500 dilution (0.1ug) (Red, Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469).

  • Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

    TSG6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate with ab267469 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267469 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate 10 ug.

    Lane 2: ab267469 IP in THP-1 (treated as above).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267469 in THP-1 (treated as above) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469).

  • Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    All lanes : Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : THP-1 (treated with /mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate
    Lane 3 : A549 (human lu carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 120,36 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

    The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

    Negative control: A549 (PMID: 19389798).

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469).

  • Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
    Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

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