Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22954-47] to GABA B Receptor 1
Suitable for: IHC-P, WB, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-GABA B Receptor 1 antibody [EPR22954-47]
See all GABA B Receptor 1 primary antibodies
Description

Rabbit monoclonal [EPR22954-47] to GABA B Receptor 1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain, Human cerebellum, Mouse cerebellum and Rat brain lysates. IHC-P: Mouse cerebellum, Rat cerebellum, Human cerebellum and Human liver tissues. IHC-Fr: Mouse cerebellum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22954-47
Isotype

IgG
Research areas

Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

All lanes : Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 108 kDa
Observed band size: 95/108 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

All the lysates were unboiled.

Skeletal muscle is a low-expression tissue.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26033241, 10996466).

Exposure time: Lanes 1-2: 75 seconds Lanes 3-4: 3 minutes.
Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

All lanes : Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lane 3 : Human skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 108 kDa
Observed band size: 95/108 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

All the lysates were unboiled.

Skeletal muscle is a low-expression tissue. T

he expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26033241, 10996466).

Exposure time: Lane 1 3 minutes Lanes 2-3: 50 seconds.
Immunohistochemistry (Frozen sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining on mouse cerebellar cortex (PMID: 22107761) is observed. Nuclear counterstain DAPI.

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com