Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-G-CSF antibody [7E4F7] (ab204989)

Key features and details

  • Mouse monoclonal [7E4F7] to G-CSF
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-G-CSF antibody [7E4F7]
    See all G-CSF primary antibodies

  • Description

    Mouse monoclonal [7E4F7] to G-CSF

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human G-CSF aa 1 to the C-terminus. (Purified from E.coli).
    Database link: P09919

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human G-CSF (aa1-207) recombinant protein; G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate; Human esophageal cancer tissue; HeLa and A549 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    7E4F7

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)
    Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)
    Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution + Human G-CSF (aa1-207) recombinant protein

    Predicted band size: 22 kDa



    Expected MWt is 48.2 kDa.

  • Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)
    Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)
    All lanes : Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (ab204989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (ab204989)

    Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling G-CSF with ab204989 at 1/200 dilution with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)
    Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)

    Immunofluorescent analysis of A549 cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)
    Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)

    Immunofluorescent analysis of HeLa cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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