Anti-G-CSF antibody [7E4F7] (ab204989)
![Anti-G-CSF antibody [7E4F7] (ab204989)](https://www.abcam.com/ps/products/204/ab204989/Images/ab204989-247632-anti-g-csf-antibody-7e4f7-western-blot.jpg "Anti-G-CSF antibody [7E4F7] (ab204989)")
Key features and details
- Mouse monoclonal [7E4F7] to G-CSF
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-G-CSF antibody [7E4F7]
See all G-CSF primary antibodies -
Description
Mouse monoclonal [7E4F7] to G-CSF -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human G-CSF aa 1 to the C-terminus. (Purified from E.coli).
Database link: P09919 -
Positive control
- Human G-CSF (aa1-207) recombinant protein; G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate; Human esophageal cancer tissue; HeLa and A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7E4F7 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution + Human G-CSF (aa1-207) recombinant protein
Predicted band size: 22 kDaExpected MWt is 48.2 kDa.
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![Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)](https://www.abcam.com/ps/products/204/ab204989/Images/ab204989-247631-anti-g-csf-antibody-7e4f7-western-blot.jpg)
Western blot - Anti-G-CSF antibody [7E4F7] (ab204989)All lanes : Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 22 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (ab204989)](https://www.abcam.com/ps/products/204/ab204989/Images/ab204989-247630-anti-g-csf-antibody-7e4f7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody [7E4F7] (ab204989)Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling G-CSF with ab204989 at 1/200 dilution with DAB staining.
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![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)](https://www.abcam.com/ps/products/204/ab204989/Images/ab204989-247629-anti-g-csf-antibody-7e4f7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)Immunofluorescent analysis of A549 cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)](https://www.abcam.com/ps/products/204/ab204989/Images/ab204989-247628-anti-g-csf-antibody-7e4f7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [7E4F7] (ab204989)Immunofluorescent analysis of HeLa cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
