Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXP3 antibody [mAbcam 450] (ab450)

Key features and details

  • Mouse monoclonal [mAbcam 450] to FOXP3
  • Suitable for: IHC-Fr, ICC, IHC-P, WB
  • Reacts with: Human, African green monkey
  • Isotype: IgG3

Overview

  • Product name

    Anti-FOXP3 antibody [mAbcam 450]
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [mAbcam 450] to FOXP3

  • Host species

    Mouse

  • Specificity

    Detects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.

  • Tested Applications & Species

    Application Species
    ICC
    African green monkey
    IHC-Fr
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: Q9BZS1
    (Peptide available as ab16809)

  • Positive control

    • IHC-P: FFPE Human tonsil tissue sections. IHC-Fr: Human tonsil tissue sections.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    All lanes : Anti-FOXP3 antibody [mAbcam 450] (ab450) at 1 µg/ml

    Lane 1 : HEK293 cells overexpressing human FOXP3
    Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa


    Exposure time: 45 seconds
  • Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450) This image is courtesy of Alison Banham and Bridget Fox, University of Oxford

    Clone mAbcam 450 used in immunocytochemistry on paraffin embedded transfected COS-1 cytospins.

    1: FOXP1 transfectant
    2: FOXP2 transfectant
    3: FOXP3 transfectant
    4: FOXP4 transfectant

    Strong staining can be seen on the FOXP3 transfectant, with very weak staining on the FOXP1 transfectant and no staining is seen with the other FOXP protein transfectants. This shows that the antibody is largely specific for FOXP3 when used with paraffin embedded cells.

    These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

    IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab450, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450) This image is courtesy of Alison Banham and Bridget Fox, University of Oxford

    Clone mAbcam 450 used in immunocytochemistry on transfected COS-1 cytospins fixed with acetone for 10 mins.

    1: FOXP1 transfectant
    2: FOXP2 transfectant
    3: FOXP3 transfectant
    4: FOXP4 transfectant

    Staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants, confirming the specificity of this antibody in immunocytochemistry.

    These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

    IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab450, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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