Anti-FAM172A antibody (ab121364)
Key features and details
- Rabbit polyclonal to FAM172A
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FAM172A antibody -
Description
Rabbit polyclonal to FAM172A -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human FAM172A aa 1-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8WUF8 -
Positive control
- ICC: U-251 MG cells; IHC-P: Human cerebral cortex, kidney, testis, and stomach tissues; WB: U-87 MG, NIH/3T3 and NBT-II cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM172A antibody (ab121364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling FAM172A with ab121364 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-FAM172A antibody (ab121364)Immunocytochemistry analysis of U-251 MG (human brain glioma cell line) cells labelling FAM172A with ab121364 at 2 µg/mL.
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Western blot - Anti-FAM172A antibody (ab121364)All lanes : Anti-FAM172A antibody (ab121364) at 0.4 µg/ml
Lane 1 : NIH-3T3 cell lysate
Lane 2 : NBT-II cell lysate
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Western blot - Anti-FAM172A antibody (ab121364)All lanes : Anti-FAM172A antibody (ab121364)
Lane 1 : U-87MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA
Lane 2 : U-87MG cells transfected with target specific siRNA probe #1
Lane 3 : U-87MG cells transfected with target specific siRNA probe #2Remaining relative intensity is presented.
Loading control: Anti-PPIB
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM172A antibody (ab121364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FAM172A with ab121364 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM172A antibody (ab121364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling FAM172A with ab121364 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM172A antibody (ab121364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling FAM172A with ab121364 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.