Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20021] to Fibroblast activation protein, alpha - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free
    See all Fibroblast activation protein, alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR20021] to Fibroblast activation protein, alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: WI-38, U-87 MG, HFF-1 and IMR-90 whole cell lysates. IHC-P: Human breast cancer, colon cancer, ovarian carcinoma and pancreatic cancer tissues; Mouse heart tissue.

  • General notes

    ab271976 is the carrier-free version of ab207178. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Paraformaldehyde-fixed tween-20 permeabilized Mouse heart tissue section stained for Fibroblast activation protein, alpha using ab207178 at 1/100 dilution (12 hour incubation 23ºC) followed by a Goat Polyclonal Alexa Fluor® 488 antibody. Endogenous peroxidase was blocked with 3% hydrogen peroxide in ddH2O for 15 min at RT.

    Antigen retrieval: Heat mediated - Buffer/Enzyme Used: EDTA pH 9

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Formalin-fixed, paraffin-embedded human colon cancer tissue labeling Fibroblast activation protein, alpha using ab207178 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retieval using Citrate, pH 6.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of formaldehyde fixed paraffin embedded human Ovarian carcinoma tissue sectioins labeling Fibroblast activation protein with ab207178 at 1/500. Biotin conjugated goat anti-rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human breast cancer is observed [PMID:18628473].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human pancreas cancer is observed [PMID:2371645].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human pancreas [PMID:22371645].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 9139873].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207178).
  • Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)
    Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

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