Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20021] to Fibroblast activation protein, alpha
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Fibroblast activation protein, alpha antibody [EPR20021]
See all Fibroblast activation protein, alpha primary antibodies
Description

Rabbit monoclonal [EPR20021] to Fibroblast activation protein, alpha
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: WI-38, U-87 MG, HFF-1 and IMR-90 whole cell lysates. IHC-P: Human breast cancer, colon cancer, ovarian carcinoma and pancreatic cancer tissues; Mouse heart tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20021
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) Courtesy of an anonymous abreview

Formalin-fixed, paraffin-embedded human colon cancer tissue labeling Fibroblast activation protein, alpha using ab207178 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retieval using Citrate, pH 6.0.
Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

All lanes : Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) at 1/1000 dilution

Lane 1 : WI-38 (Human fetal lung fibroblast cell line) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 88 kDa
Observed band size: 95 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight is consistent with the literature.

Negative control: Human fetal liver (PMID: 10593948, PMID: 9139873).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) This image is of an Abreciew submitted byCarl Hobbs.

Immunohistochemical analysis of formaldehyde fixed paraffin embedded human Ovarian carcinoma tissue sectioins labeling Fibroblast activation protein with ab207178 at 1/500. Biotin conjugated goat anti-rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid.
See Abreview
Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

All lanes : Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) at 1/1000 dilution

Lane 1 : HFF-1 (Human forskin fibroblast cell line) whole cell lysate
Lane 2 : IMR-90 (Human lung fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 88 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.

The molecular weight is consistent with the literature (PMID: 10593948).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) This image was courtesy of an anonymous AbReview

Paraformaldehyde-fixed tween-20 permeabilized Mouse heart tissue section stained for Fibroblast activation protein, alpha using ab207178 at 1/100 dilution (12 hour incubation 23ºC) followed by a Goat Polyclonal Alexa Fluor® 488 antibody. Endogenous peroxidase was blocked with 3% hydrogen peroxide in ddH2O for 15 min at RT.

Antigen retrieval: Heat mediated - Buffer/Enzyme Used: EDTA pH 9
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human pancreas cancer is observed [PMID:2371645].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human breast cancer is observed [PMID:18628473].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: no staining on human pancreas [PMID:22371645].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: no staining on human liver [PMID: 9139873].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

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