Human FAP ELISA Kit (ab256404)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14.96 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FAP ELISA Kit
See all Fibroblast activation protein, alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.9% Inter-assay Sample n Mean SD CV% Serum 3 9.8% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14.96 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 90% - 102% Cell culture media 93 91% - 96% Hep Plasma 97 91% - 101% EDTA Plasma 103 96% - 108% Cit plasma 90 82% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human FAP ELISA Kit (ab256404) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAP protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FAP with 14.96 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
FAP (fibroblast activation protein-alpha; also known as seprase) is a 86.3 kD type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue. FAP may play roles in tumor invasion, tissue remodeling, and wound repair. Human FAP shares 90.1%, 89.5%, 93.9%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FAP Capture Antibody 1 x 600µl 10X Human FAP Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human FAP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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Alternative names
- 170 kDa melanoma membrane bound gelatinase
- 170 kDa melanoma membrane-bound gelatinase
- DPPIV
see all -
Database links
- Entrez Gene: 2191 Human
- Omim: 600403 Human
- SwissProt: Q12884 Human
- Unigene: 654370 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human FAP standard curve in Sample Diluent NS.The FAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FAP in human serum and plasma samples.The concentrations of FAP were measured in duplicates, interpolated from the FAP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 29.25 ng/mL in serum, 24.36 ng/mL in plasma (citrate), 26.29 ng/mL in plasma (EDTA), and 24.01 ng/mL in plasma (heparin).
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2.5% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 53.12 ng/mL with a range of 20.47 – 101.21 ng/mL.
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Sandwich ELISA - Human FAP ELISA Kit (ab256404)To learn more about the advantages of recombinant antibodies see here.
