Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16642] to Bcl-XL - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free
    See all Bcl-XL primary antibodies

  • Description

    Rabbit monoclonal [EPR16642] to Bcl-XL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, Jurkat, HeLa or K562 whole cell lysate. Mouse brain, heart, kidney, spleen. Rat brain, heart, kidney, spleen. C6, RAW 264.7, PC-12 or NIH/3T3 whole cell lysate. IHC-P: Human kidney, mouse colon, rat testis.

  • General notes

    Ab223547 is the carrier-free version of ab178844. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223547 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Flow Cytometry - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa cells labelling Bcl-XL with purified ab178844 at a dilution of 1/100 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of mouse colon tissue is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of testis is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:5% NFDM/TBST.
  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution developed using the ECL technique

    Predicted band size: 26 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse heart
    Lane 3 : Mouse kidney
    Lane 4 : Mouse spleen
    Lane 7 : Rat kidney
    Lane 8 : Rat spleen
    Lane 9 : C6 whole cell lysates
    Lane 10 : Raw264.7 whole cell lysates
    Lane 11 : PC-12 whole cell lysates
    Lane 12 : NIH/3T3 whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Rat testis tissue.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of testis is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

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