Anti-FGF21 antibody (ab64857)
Key features and details
- Rabbit polyclonal to FGF21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF21 antibody
See all FGF21 primary antibodies -
Description
Rabbit polyclonal to FGF21 -
Host species
Rabbit -
Specificity
Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)]) -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.
Read Abcam's proprietary immunogen policy (Peptide available as ab66562.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).Target
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Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 26291 Human
- Entrez Gene: 56636 Mouse
- Entrez Gene: 170580 Rat
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- SwissProt: Q9JJN1 Mouse
- Unigene: 283015 Human
- Unigene: 143736 Mouse
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Alternative names
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
Images
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Western blot - Anti-FGF21 antibody (ab64857)Anti-FGF21 antibody (ab64857) at 1 µg/ml +
Recombinant human FGF21 protein (Active) (ab54141) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.
References (13)
ab64857 has been referenced in 13 publications.
- Zhou L et al. MicroRNA-100-5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727-738 (2019). PubMed: 30535435
- Seo YJ et al. Ishige okamurae Extract Ameliorates the Hyperglycemia and Body Weight Gain of db/db Mice through Regulation of the PI3K/Akt Pathway and Thermogenic Factors by FGF21. Mar Drugs 17:N/A (2019). PubMed: 31323977
- Pan Y et al. Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling-dependent manner. J Cell Mol Med N/A:N/A (2018). PubMed: 30461198
- Zhang Y et al. TFEB-dependent induction of thermogenesis by the hepatocyte SLC2A inhibitor trehalose. Autophagy 14:1959-1975 (2018). PubMed: 29996716
- Zhang J et al. Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency. Exp Ther Med 15:3189-3196 (2018). PubMed: 29545834
Images
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Western blot - Anti-FGF21 antibody (ab64857)Anti-FGF21 antibody (ab64857) at 1 µg/ml +
Recombinant human FGF21 protein (Active) (ab54141) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.
