Human aFGF ELISA Kit (acid FGF) (ab193691)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 12 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human aFGF ELISA Kit (acid FGF)
See all FGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
12 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122.5 105% - 131% Plasma 91.51 78% - 103% Cell culture media 76.17 66% - 87% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s aFGF (acid FGF) Human ELISA Kit (ab193691) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human aFGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human aFGF coated on a 96-well plate. Standards and samples are pipetted into the wells and aFGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human aFGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of aFGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Human aFGF detection antibody (lyophilized) 2 vials Human aFGF standards (lyophilized) 2 vials Pre-coated Human aFGF Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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Alternative names
- Acidic fibroblast growth factor
- aFGF
- Beta endothelial cell growth factor
see all -
Database links
- Entrez Gene: 2246 Human
- Omim: 131220 Human
- SwissProt: P05230 Human
- Unigene: 483635 Human
Images
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Typical Standard Curve
Standard Curve using ab193691 aFGF (acid FGF) Human ELISA Kit.