Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF21 antibody [EPR8314(2)] (ab171941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8314(2)] to FGF21
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FGF21 antibody [EPR8314(2)]
    See all FGF21 primary antibodies

  • Description

    Rabbit monoclonal [EPR8314(2)] to FGF21

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.

    Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327).  For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FGF21 aa 1-100. The exact sequence is proprietary.
    Database link: Q9NSA1

  • Positive control

    • Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. WB: Human fetal liver lysate, mouse spleen, rat spleen. IHC-P: Human stomach

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Rat spleen tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Western blot - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-FGF21 antibody [EPR8314(2)] (ab171941)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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