Recombinant human FGF2 protein (Active) (ab155734)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: ELISA, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human FGF2 protein (ab155734)Human FGF basic (Tag Free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
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ELISA - Recombinant Human FGF2 protein (ab155734)Immobilized ab155734 at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag (ab220538) with a linear range of 4-125 ng/mL
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ELISA - Recombinant Human FGF2 protein (ab155734)Immobilized ab155734 at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag with a linear range of 1-39 ng/mL.
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Functional Studies - Recombinant human FGF2 protein (ab155734)Immobilized ab155734 on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200).
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Functional Studies - Recombinant human FGF2 protein (ab155734)Loaded Biotinylated Heparin on SA Biosensor can bind ab155734 with an affinity constant of 1.88nM as determined in BLI assay (ForteBio Octet Red96e).
