Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)
![Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)](https://www.abcam.com/ps/products/223/ab223544/Images/ab223544-282798-ab207321WB.jpg "Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19989] to FGF1 - Low endotoxin, Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free
See all FGF1 primary antibodies -
Description
Rabbit monoclonal [EPR19989] to FGF1 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney, fetal brain and glioma lysates; U-87 MG and Neuro-2a whole cell lysates; Mouse spinal cord, heart and kidney lysates; Rat heart lysate.
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General notes
ab223544 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19989 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)All lanes : Anti-FGF1 antibody [EPR19989] (ab207321)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 17 kDa
Observed band size: 16,18 kDa why is the actual band size different from the predicted?This WB data was generated using the same anti-FGF1 antibody clone [EPR19989] in a different buffer formulation (cat# ab207321).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 second; Lane 2/4: 5 seconds; Lane 3: 3 seconds.
The observed molecular weight is consistent with the literature (PMID: 16091423).
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![Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)](https://www.abcam.com/ps/products/223/ab223544/Images/ab223544-2-benefits-of-recombinant-antibodies.png)
Anti-FGF1 antibody [EPR19989] - Low endotoxin, Azide free (ab223544)
