Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19989] to FGF1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FGF1 antibody [EPR19989] - BSA and Azide free
    See all FGF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19989] to FGF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, fetal brain and glioma lysates; U-87 MG and Neuro-2a whole cell lysates; Mouse spinal cord, heart and kidney lysates; Rat heart lysate.

  • General notes

    ab271978 is the carrier-free version of ab207321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
    Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
    All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lanes 1 & 5 : Human glioma lysate
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 2-3 & 5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 17 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207321).

  • Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
    Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
    All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 17 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.

    The observed molecular weight is consistent with the literature (PMID: 16091423).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207321).

  • Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
    Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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