Anti-SPRY3 antibody (ab233424)
Key features and details
- Rabbit polyclonal to SPRY3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SPRY3 antibody -
Description
Rabbit polyclonal to SPRY3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human SPRY3 aa 50 to the C-terminus. N-terminal Tags. (Expressed in E.coli).
Database link: O43610 -
Positive control
- WB: Rat brain cerebrum tissue lysate - total protein (ab4033), Human lung lysate; Rat and mouse cerebrum lysate; Recombinant human SPRY3 protein. IHC-P: Human liver cancer, liver and kidney cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPRY3 antibody (ab233424)Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Rat cerebrum lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)Formalin-fixed, paraffin embedded human liver tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)Formalin-fixed, paraffin embedded human liver cancer tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)Formalin-fixed, paraffin embedded human renal cancer tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-SPRY3 antibody (ab233424)Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Mouse cerebrum lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
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Western blot - Anti-SPRY3 antibody (ab233424)Anti-SPRY3 antibody (ab233424) at 3 µg/ml + Human lung lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
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Western blot - Anti-SPRY3 antibody (ab233424)Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Recombinant human SPRY3 protein